Glucocorticoids rapidly inhibit oxytocin-stimulated adrenocorticotropin release from rat anterior pituitary cells, without modifying intracellular calcium transients

Glucocorticoid hormones suppress the secretion of ACTH evoked by secretagogues such as CRF and arginine vasopressin. In this study, we investigated the effects of glucocorticoids on ACTH release induced by oxytocin (OT) and on intracellular free calcium ion levels in corticotropes prepared from the adenohypophyses of female Wistar rats. Pulsatile additions of physiological concentration of OT (10 nM) to superfused anterior pituitary cells caused pulsatile ACTH release about 4-fold above basal secretion with similar peak amounts of ACTH during subsequent OT pulses. Exposure of the cells to corticosterone (100 nM) or to a selective glucocorticoid receptor agonist RU 28362 (100 nM) for 30 min suppressed OT-stimulated but not basal ACTH release by approximately 60%. Inhibition gradually disappeared during subsequent pulses of OT in the absence of corticosterone. Pretreatment with the selective antagonist RU 38486 (1 microM) completely blocked the inhibitory effect of corticosterone on OT-induced ACTH secretion. Changes in free cytosolic calcium levels in single cultured pituitary cells were measured using the calcium indicator Fura-2. OT caused calcium transients in corticotropes, which were identified by immunocytochemistry. They responded in a similar manner to a second OT stimulus when preincubated for 30 min with corticosterone (1 microM) or with RU 28362 (1 microM). Our data indicate that glucocorticoids, via glucocorticoid receptors, rapidly inhibit OT-stimulated ACTH secretion by corticotropes without affecting intracellular calcium transients due to OT. Therefore, we conclude that rapid inhibition of ACTH release by glucocorticoids interferes with cellular signal transduction beyond the step of calcium mobilization

Residual Stresses in Layered Manufacturing

Layered Manufacturing processes accumulate residual stresses during materialbuildup. These stresses may cause part warping and layer delamination. This paper presents work done on investigating residual stress accumulation andp(i,rt distortion of Layered Manufactured artifacts. A simple analyticaLmodel was developed and used to determine how the number of layers and the layer thickness influences part warping. Resllits show that thin layers produce lower part deflection as compared with depositing fewer and thicker layers. In addition to the analytical work, a finite element model wasdeveloped and used to illvestigate the deposition pattern's influence on. the part deflection. Finite element model and corresponding experimental analysis showed that the geometry of the deposition pattern significantly affects the resulting part distortion. This finite element model was also used to investigate an inter-layer surface defect,. known as the Christmas Thee Step, that is associated with Shape Deposition Manufacturing. Results indicate that the features of this defect are influenced only by the material deposited close. to the part·surface and the particular material deposited. The step is not affected by the deposition pattern.Mechanical Engineerin

Oxytocin at physiological concentrations evokes adrenocorticotropin (ACTH) release from corticotrophs by increasing intracellular free calcium mobilized mainly from intracellular stores. Oxytocin displays synergistic or additive effects on ACTH-releasing factor or arginine vasopressin-induced ACTH secretion, respectively

The potency of oxytocin (OT) in evoking ACTH secretion by isolated, superfused rat adenohypophyseal corticotrophs and its enhancement by CRF and arginine vasopressin (AVP) were analyzed. Each secretagogue effectively released ACTH from adenohypophyseal cells when added separately in pulsatile fashion in physiological concentrations based on hypophyseal portal blood (OT, 10 nM; AVP, 0.5 nM; CRF, 0.1 nM). OT released ACTH at concentrations as low as 1 nM. Moreover, a dose- response relationship up to 10 microM was revealed. Combinations of a constant amount of CRF (0.1 nM) with increasing concentrations of OT exerted a synergistic effect on ACTH release. In contrast, OT given in various concentrations in combination with AVP (0.5 nM) produced an additive effect on ACTH release. To study the mechanism of action of OT on ACTH secretion, cytosolic free calcium levels in single pituitary cells exposed to OT or AVP were measured using the calcium-sensitive fluorescent indicator Fura-2. Corticotrophs among mixed adenohypophyseal cell types in the primary cultures were identified by immunocytochemistry. More than 500 cells were individually stimulated with OT or AVP. Basal cytosolic free calcium levels ranged between 80- 130 nM free calcium. The addition of 100 nM OT or 1 microM AVP increased the cytosolic free calcium concentration within 3 sec to values ranging from 500-800 nM. An increase in intracellular calcium ranging from 200-500 nM due to OT could still be observed after extracellular calcium depletion. Taken together, our data demonstrate that physiological concentrations of OT stimulate ACTH secretion, independent of the other ACTH secretagogues, by mobilizing calcium mainly from intracellular stores

Field measurements of a swell band, shore normal, flux divergence reversal

Submitted in partial fulfillment of the requirements for the degree of Master of Science at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution June 2011Throughout this thesis we will discuss the theoretical background and empirical observation of a swell band shore normal flux divergence reversal. Specifically, we will demonstrate the existence and persistence of the energy flux divergence reversal in the nearshore region of Atchafalaya Bay, Gulf of Mexico, across storms during the March through April 2010 deployment. We will show that the swell band offshore component of energy flux is rather insignificant during the periods of interest, and as such we will neglect it during the ensuing analysis. The data presented will verify that the greatest flux divergence reversal is seen with winds from the East to Southeast, which is consistent with theories which suggest shoreward energy flux as well as estuarine sediment transport and resuspension prior to passage of a cold front. Employing the results of theoretical calculations and numerical modeling we will confirm that a plausible explanation for this phenomena can be found in situations where temporally varying wind input may locally balance or overpower bottom induced dissipation, which may also contravene the hypothesis that dissipation need increase shoreward due to nonlinear wave-wave interactions and maturation of the spectrum. Lastly, we will verify that the data presented is consistent with other measures collected during the same deployment in the Atchafalaya Bay during March - April 2010

Neutral Current π0\pi^0 Production in MiniBooNE

This paper describes the analysis used to determine the neutral current $\pi^0$ production in MiniBooNE in bins of momentum. Additionally, a measurement of the relative coherent production of $\pi^0$s is discussed. The coherent production rate is found to be (19.5 $\pm$1.1 (stat) $\pm$2.5 (sys))% of the total exclusive neutral current $\pi^0$ production rate.Comment: Prepared for the Proceedings of Neutrino Interactions 200

Interpreting experimental bounds on D^0 - \bar{D^0} mixing in the presence of CP violation

We analyse the most recent experimental data regarding D^0 - \bar{D^0} mixing, allowing for CP violation. We focus on the dispersive part of the mixing amplitude, M^D_{12}, which is sensitive to new physics contributions. We obtain a constraint on the mixing amplitude: |M^D_{12}| < 6.2\times 10^{-11} MeV at 95% C.L. . This constraint is weaker by a factor of about three than the one which is obtained when no CP violation is assumed.Comment: 9 pages, revtex4; One reference updated, one reference added, footnote 3 correcte

Movement as a specific stimulus for prey catching behaviour in rhinolophid and hipposiderid bats

1. The echolocating 'long CF/FM-bat'Rhinolophus rouxi and the 'short CF/FM-bats'Hipposideros bicolor andHipposideros speoris were tested for catching responses to moving and non-moving targets. 2. Under our experimental conditions (freshly caught caged bats in a natural environment)Rhinolophus rouxi and Hipposideros speoris only responded to insects of any sort that were beating their wings. The bats showed no reactions whatsoever to nonmoving insects or those walking on the floor or the sides of the cage. 3. Hipposideros bicolor responded in the same way as the above species to wingbeating insects but in addition also attacked walking insects. In 27 presentations 15 walking insects were caught (Fig. 2). 4. Rhinolophus rouxi, Hipposideros speoris and Hipposideros bicolor also detected, approached and seized tethered cockroaches hanging from the ceiling when these were vibrating up and down (Fig. 3). This indicates that any oscillating movement and not specific aspects of wing beating were the key releasers for catching behaviour in all three species. However, a wing beating insect is strongly preferred over a vibrating one in all three species (Fig. 4). 5. Rhinolophus rouxi, Hipposideros speoris and Hipposideros bicolor attacked and seized a dead bait when it was associated with a wing beating device (Fig. 1). All three species responded effectively to beat frequencies as low as 10 beats/s (peak-to-peak amplitude of the wing excursion 20 mm). For lower frequencies the response rates rapidly deteriorated (Fig. 5). 6. Horseshoe bats no longer responded to wing beats of 5 beats/s when the wing beat amplitude was 2 to 1 mm or to wing beats of 2 to 1 beats/s when the amplitude was 3 mm or lower (Fig. 6). This suggests that the speed of the wing is a critical parameter. From these data we infer that the threshold for the catching responses is at a wing speed of about 2 to 1 cm/s. 7. In horseshoe bats (experimental tests) and the two hipposiderid species (behavioural observations) one single wing beat was enough to elicit a catching response (Fig. 8). 8. It is concluded that 'long' and 'short' CF/ FM-bats feature a similar responsiveness to fluttering targets. The sensitivity to oscillating movements is considered as an effective detection mechanism for any sort of potential prey

Social Conditions as Fundamental Causes of Disease

Over the last several decades, epidemiological studies have been enormously successful in identifying risk factors for major diseases. However, most of this research has focused attention on risk factors that are relatively proximal causes of disease such as diet, cholesterol level, exercise and the like. We question the emphasis on such individually-based risk factors and argue that greater attention must be paid to basic social conditions if health reform is to have its maximum effect in the time ahead. There are two reasons for this claim. First we argue that individually-based risk factors must be contextualized, by examining what puts people at risk of risks, if we are to craft effective interventions and improve the nation's health. Second, we argue that social factors such as socioeconomic status and social support are likely 'fundamental causes" of disease that, because they embody access to important resources, affect multiple disease outcomes throughmultiple mechanisms, and consequently maintain an association with disease even when intervening mechanisms change. Without careful attention to these possibilities, we run the risk of imposing individually-based intervention strategies that are ineffective and of missing opportunities to adopt broad-based societal interventions that could produce substantial health benefits for our citizen